1,2-α-d-mannosidase from Penicillium citrinum: molecular and enzymic properties of two isoenzymes
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چکیده
منابع مشابه
1 , 2 - i - D - Mannosidase from Peniciiium citrinum : molecular and enzymic properties of two isoenzymes
Two isoforms of acidic 1,2-a-D-mannosidases have been isolated from culture filtrate of Penicillium citrinum. The pl values of the two forms, designated 1,2-a-mannosidase la and lb, were 4.6 and 4.7 respectively. Isoenzymes Ia and Ib exhibited the same molecular mass which was determined to be 53 kDa by SDS/ PAGE and 54 kDa by gel-permeation chromatography. Enzymes Ia and lb hydrolysed yeast ma...
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آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1993
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2900349